Rapid isolation of respiring skeletal muscle mitochondria using nitrogen cavitation

Methods of isolating mitochondria commonly utilise mechanical force and shear stress to homogenize tissue followed by purification by multiple rounds of ultracentrifugation. Existing protocols can be time-consuming with some physically impairing integrity of the sensitive mitochondrial double membra...

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Detalles Bibliográficos
Autores principales: Younis, Awais Z., Lavery, Gareth G., Christian, Mark, Doig, Craig L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027933/
https://www.ncbi.nlm.nih.gov/pubmed/36960150
http://dx.doi.org/10.3389/fphys.2023.1114595

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027933/
https://www.ncbi.nlm.nih.gov/pubmed/36960150
http://dx.doi.org/10.3389/fphys.2023.1114595

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