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Rapid isolation of respiring skeletal muscle mitochondria using nitrogen cavitation
Methods of isolating mitochondria commonly utilise mechanical force and shear stress to homogenize tissue followed by purification by multiple rounds of ultracentrifugation. Existing protocols can be time-consuming with some physically impairing integrity of the sensitive mitochondrial double membra...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027933/ https://www.ncbi.nlm.nih.gov/pubmed/36960150 http://dx.doi.org/10.3389/fphys.2023.1114595 |