New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference

[Image: see text] CRISPR interference (CRISPRi) screening has been used for identification of target genes related to specific phenotypes using single-molecular guide RNA (sgRNA) libraries. In CRISPRi screening, the sizes of random sgRNA libraries contained with the original target recognition seque...

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Detalles Bibliográficos
Autores principales: Jeong, Song Hee, Kim, Hyun Ju, Lee, Sang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028695/
https://www.ncbi.nlm.nih.gov/pubmed/36787424
http://dx.doi.org/10.1021/acssynbio.2c00595

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028695/
https://www.ncbi.nlm.nih.gov/pubmed/36787424
http://dx.doi.org/10.1021/acssynbio.2c00595

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