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New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference
[Image: see text] CRISPR interference (CRISPRi) screening has been used for identification of target genes related to specific phenotypes using single-molecular guide RNA (sgRNA) libraries. In CRISPRi screening, the sizes of random sgRNA libraries contained with the original target recognition seque...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028695/ https://www.ncbi.nlm.nih.gov/pubmed/36787424 http://dx.doi.org/10.1021/acssynbio.2c00595 |