A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants
Plant expression vectors are essential tools for gene functional analysis and molecular plant breeding. The gene of interest is transferred to the vector by molecular cloning technology. Nimble Cloning is a newly developed molecular cloning method with the advantages of simplicity, efficiency, and s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030367/ https://www.ncbi.nlm.nih.gov/pubmed/36352791 http://dx.doi.org/10.1016/j.xplc.2022.100471 |
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author | Yan, Pu Tuo, Decai Shen, Wentao Deng, Haida Zhou, Peng Gao, Xinzheng |
author_facet | Yan, Pu Tuo, Decai Shen, Wentao Deng, Haida Zhou, Peng Gao, Xinzheng |
author_sort | Yan, Pu |
collection | PubMed |
description | Plant expression vectors are essential tools for gene functional analysis and molecular plant breeding. The gene of interest is transferred to the vector by molecular cloning technology. Nimble Cloning is a newly developed molecular cloning method with the advantages of simplicity, efficiency, and standardization. In this study, we developed a “pNC” vector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in plants. These vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble Cloning. We demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes, including the study of gene ectopic expression, protein subcellular localization, protein–protein interaction, gene silencing (RNAi), virus-induced gene silencing, promoter activity, and CRISPR–Cas9-mediated genome editing. The “pNC” vector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics. |
format | Online Article Text |
id | pubmed-10030367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100303672023-03-23 A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants Yan, Pu Tuo, Decai Shen, Wentao Deng, Haida Zhou, Peng Gao, Xinzheng Plant Commun Resource Article Plant expression vectors are essential tools for gene functional analysis and molecular plant breeding. The gene of interest is transferred to the vector by molecular cloning technology. Nimble Cloning is a newly developed molecular cloning method with the advantages of simplicity, efficiency, and standardization. In this study, we developed a “pNC” vector system that contains 55 Nimble Cloning-compatible vectors for functional analysis of genes in plants. These vectors contain the NC frame flanked by unique adapters for one-step and standardized Nimble Cloning. We demonstrate that the pNC vectors are convenient and effective for the functional analysis of plant genes, including the study of gene ectopic expression, protein subcellular localization, protein–protein interaction, gene silencing (RNAi), virus-induced gene silencing, promoter activity, and CRISPR–Cas9-mediated genome editing. The “pNC” vector system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant functional genomics. Elsevier 2022-11-09 /pmc/articles/PMC10030367/ /pubmed/36352791 http://dx.doi.org/10.1016/j.xplc.2022.100471 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Article Yan, Pu Tuo, Decai Shen, Wentao Deng, Haida Zhou, Peng Gao, Xinzheng A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title | A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title_full | A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title_fullStr | A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title_full_unstemmed | A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title_short | A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants |
title_sort | nimble cloning-compatible vector system for high-throughput gene functional analysis in plants |
topic | Resource Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030367/ https://www.ncbi.nlm.nih.gov/pubmed/36352791 http://dx.doi.org/10.1016/j.xplc.2022.100471 |
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