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Optimization of a high-throughput nanofluidic real-time PCR to detect and quantify of 15 bacterial species and 92 Streptococcus pneumoniae serotypes

Sensitive tools for detecting concurrent colonizing pneumococcal serotypes are needed for detailed evaluation of the direct and indirect impact of routine pneumococcal conjugate vaccine (PCV) immunization. A high-throughput quantitative nanofluidic real-time PCR (Standard BioTools ‘Fluidigm’) reacti...

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Detalles Bibliográficos
Autores principales: Downs, Sarah L., Madhi, Shabir. A., van der Merwe, Lara, Nunes, Marta. C., Olwagen, Courtney P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030628/
https://www.ncbi.nlm.nih.gov/pubmed/36944704
http://dx.doi.org/10.1038/s41598-023-31820-4