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Multiplex measurement of protein–peptide dissociation constants using dialysis and mass spectrometry
We propose a high‐throughput method for quantitatively measuring hundreds of protein–peptide binding affinities in parallel. In this assay, a solution of protein is dialyzed into a buffer containing a pool of potential binding peptides, such that upon equilibration the relative abundance of a peptid...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031237/ https://www.ncbi.nlm.nih.gov/pubmed/36823715 http://dx.doi.org/10.1002/pro.4607 |