Protein Pull-down Assay Using HiBiT-tag-dependent Luciferase Activity Measurement

Co-immunoprecipitation or pull-down assays are frequently used to analyze protein–protein interactions. In these experiments, western blotting is commonly used to detect prey proteins. However, sensitivity and quantification problems remain in this detection system. Recently, the HiBiT-tag-dependent...

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Detalles Bibliográficos
Autores principales: Arakawa, Masashi, Morita, Eiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031519/
https://www.ncbi.nlm.nih.gov/pubmed/36968438
http://dx.doi.org/10.21769/BioProtoc.4640

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031519/
https://www.ncbi.nlm.nih.gov/pubmed/36968438
http://dx.doi.org/10.21769/BioProtoc.4640

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