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Protein Pull-down Assay Using HiBiT-tag-dependent Luciferase Activity Measurement
Co-immunoprecipitation or pull-down assays are frequently used to analyze protein–protein interactions. In these experiments, western blotting is commonly used to detect prey proteins. However, sensitivity and quantification problems remain in this detection system. Recently, the HiBiT-tag-dependent...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Bio-Protocol
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031519/ https://www.ncbi.nlm.nih.gov/pubmed/36968438 http://dx.doi.org/10.21769/BioProtoc.4640 |