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Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues

Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2–(3)H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue processi...

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Detalles Bibliográficos
Autores principales: Zhao, Meng, Wat, Lianna W., Svensson, Katrin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031528/
https://www.ncbi.nlm.nih.gov/pubmed/36933224
http://dx.doi.org/10.1016/j.xpro.2023.102179