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Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues
Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2–(3)H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue processi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031528/ https://www.ncbi.nlm.nih.gov/pubmed/36933224 http://dx.doi.org/10.1016/j.xpro.2023.102179 |