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High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages

Regulation of bioenergetics and cell death are pivotal mitochondrial functions determining the responses of macrophages to infection. Here, we provide a protocol to investigate mitochondrial functions during infection of macrophages by intracellular bacteria. We describe steps for quantifying mitoch...

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Detalles Bibliográficos
Autores principales: Dramé, Mariatou, Garcia-Rodriguez, Francisco Javier, Buchrieser, Carmen, Escoll, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031535/
https://www.ncbi.nlm.nih.gov/pubmed/36933221
http://dx.doi.org/10.1016/j.xpro.2023.102175