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High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages
Regulation of bioenergetics and cell death are pivotal mitochondrial functions determining the responses of macrophages to infection. Here, we provide a protocol to investigate mitochondrial functions during infection of macrophages by intracellular bacteria. We describe steps for quantifying mitoch...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031535/ https://www.ncbi.nlm.nih.gov/pubmed/36933221 http://dx.doi.org/10.1016/j.xpro.2023.102175 |