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Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p
BACKGROUND: Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression. METHODS: IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031964/ https://www.ncbi.nlm.nih.gov/pubmed/36949500 http://dx.doi.org/10.1186/s13018-023-03638-3 |
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author | Liu, Hongbo Zhao, Hongxia Huang, Yin Lei, Ming |
author_facet | Liu, Hongbo Zhao, Hongxia Huang, Yin Lei, Ming |
author_sort | Liu, Hongbo |
collection | PubMed |
description | BACKGROUND: Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression. METHODS: IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot. RESULTS: Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression. CONCLUSION: Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13018-023-03638-3. |
format | Online Article Text |
id | pubmed-10031964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-100319642023-03-23 Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p Liu, Hongbo Zhao, Hongxia Huang, Yin Lei, Ming J Orthop Surg Res Research Article BACKGROUND: Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression. METHODS: IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot. RESULTS: Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression. CONCLUSION: Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13018-023-03638-3. BioMed Central 2023-03-22 /pmc/articles/PMC10031964/ /pubmed/36949500 http://dx.doi.org/10.1186/s13018-023-03638-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Liu, Hongbo Zhao, Hongxia Huang, Yin Lei, Ming Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title | Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title_full | Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title_fullStr | Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title_full_unstemmed | Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title_short | Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p |
title_sort | circ_0002715 promotes the development of osteoarthritis through regulating lxn by sponging mir-127-5p |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031964/ https://www.ncbi.nlm.nih.gov/pubmed/36949500 http://dx.doi.org/10.1186/s13018-023-03638-3 |
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