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A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice

Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly g...

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Autores principales: Wu, Mingming, Cai, Maohong, Zhai, Rongrong, Ye, Jing, Zhu, Guofu, Yu, Faming, Ye, Shenghai, Zhang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033517/
https://www.ncbi.nlm.nih.gov/pubmed/36968383
http://dx.doi.org/10.3389/fpls.2023.1136849
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author Wu, Mingming
Cai, Maohong
Zhai, Rongrong
Ye, Jing
Zhu, Guofu
Yu, Faming
Ye, Shenghai
Zhang, Xiaoming
author_facet Wu, Mingming
Cai, Maohong
Zhai, Rongrong
Ye, Jing
Zhu, Guofu
Yu, Faming
Ye, Shenghai
Zhang, Xiaoming
author_sort Wu, Mingming
collection PubMed
description Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly grain 1 (wbg1), which has a white belly in its mature grains. The grain filling rate of wbg1 was lower than that of the wild type across the whole filling period, and the starch granules in the chalky part were oval or round and loosely arranged. Map-based cloning showed that wbg1 was an allelic mutant of FLO10, which encodes a mitochondrion-targeted P-type pentatricopeptide repeat protein. Amino acid sequence analysis found that two PPR motifs present in the C-terminal of WBG1 were lost in wbg1. This deletion reduced the splicing efficiency of nad1 intron 1 to approximately 50% in wbg1, thereby partially reducing the activity of complex I and affecting ATP production in wbg1 grains. Furthermore, haplotype analysis showed that WBG1 was associated with grain width between indica and japonica rice varieties. These results suggested that WBG1 influences rice grain chalkiness and grain width by regulating the splicing efficiency of nad1 intron 1. This deepens understanding of the molecular mechanisms governing rice grain quality and provides theoretical support for molecular breeding to improve rice quality.
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spelling pubmed-100335172023-03-24 A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice Wu, Mingming Cai, Maohong Zhai, Rongrong Ye, Jing Zhu, Guofu Yu, Faming Ye, Shenghai Zhang, Xiaoming Front Plant Sci Plant Science Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly grain 1 (wbg1), which has a white belly in its mature grains. The grain filling rate of wbg1 was lower than that of the wild type across the whole filling period, and the starch granules in the chalky part were oval or round and loosely arranged. Map-based cloning showed that wbg1 was an allelic mutant of FLO10, which encodes a mitochondrion-targeted P-type pentatricopeptide repeat protein. Amino acid sequence analysis found that two PPR motifs present in the C-terminal of WBG1 were lost in wbg1. This deletion reduced the splicing efficiency of nad1 intron 1 to approximately 50% in wbg1, thereby partially reducing the activity of complex I and affecting ATP production in wbg1 grains. Furthermore, haplotype analysis showed that WBG1 was associated with grain width between indica and japonica rice varieties. These results suggested that WBG1 influences rice grain chalkiness and grain width by regulating the splicing efficiency of nad1 intron 1. This deepens understanding of the molecular mechanisms governing rice grain quality and provides theoretical support for molecular breeding to improve rice quality. Frontiers Media S.A. 2023-03-09 /pmc/articles/PMC10033517/ /pubmed/36968383 http://dx.doi.org/10.3389/fpls.2023.1136849 Text en Copyright © 2023 Wu, Cai, Zhai, Ye, Zhu, Yu, Ye and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wu, Mingming
Cai, Maohong
Zhai, Rongrong
Ye, Jing
Zhu, Guofu
Yu, Faming
Ye, Shenghai
Zhang, Xiaoming
A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title_full A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title_fullStr A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title_full_unstemmed A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title_short A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
title_sort mitochondrion-associated ppr protein, wbg1, regulates grain chalkiness in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033517/
https://www.ncbi.nlm.nih.gov/pubmed/36968383
http://dx.doi.org/10.3389/fpls.2023.1136849
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