Cargando…
A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice
Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly g...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033517/ https://www.ncbi.nlm.nih.gov/pubmed/36968383 http://dx.doi.org/10.3389/fpls.2023.1136849 |
_version_ | 1784911007601131520 |
---|---|
author | Wu, Mingming Cai, Maohong Zhai, Rongrong Ye, Jing Zhu, Guofu Yu, Faming Ye, Shenghai Zhang, Xiaoming |
author_facet | Wu, Mingming Cai, Maohong Zhai, Rongrong Ye, Jing Zhu, Guofu Yu, Faming Ye, Shenghai Zhang, Xiaoming |
author_sort | Wu, Mingming |
collection | PubMed |
description | Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly grain 1 (wbg1), which has a white belly in its mature grains. The grain filling rate of wbg1 was lower than that of the wild type across the whole filling period, and the starch granules in the chalky part were oval or round and loosely arranged. Map-based cloning showed that wbg1 was an allelic mutant of FLO10, which encodes a mitochondrion-targeted P-type pentatricopeptide repeat protein. Amino acid sequence analysis found that two PPR motifs present in the C-terminal of WBG1 were lost in wbg1. This deletion reduced the splicing efficiency of nad1 intron 1 to approximately 50% in wbg1, thereby partially reducing the activity of complex I and affecting ATP production in wbg1 grains. Furthermore, haplotype analysis showed that WBG1 was associated with grain width between indica and japonica rice varieties. These results suggested that WBG1 influences rice grain chalkiness and grain width by regulating the splicing efficiency of nad1 intron 1. This deepens understanding of the molecular mechanisms governing rice grain quality and provides theoretical support for molecular breeding to improve rice quality. |
format | Online Article Text |
id | pubmed-10033517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100335172023-03-24 A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice Wu, Mingming Cai, Maohong Zhai, Rongrong Ye, Jing Zhu, Guofu Yu, Faming Ye, Shenghai Zhang, Xiaoming Front Plant Sci Plant Science Rice kernel quality has vital commercial value. Grain chalkiness deteriorates rice’s appearance and palatability. However, the molecular mechanisms that govern grain chalkiness remain unclear and may be regulated by many factors. In this study, we identified a stable hereditary mutant, white belly grain 1 (wbg1), which has a white belly in its mature grains. The grain filling rate of wbg1 was lower than that of the wild type across the whole filling period, and the starch granules in the chalky part were oval or round and loosely arranged. Map-based cloning showed that wbg1 was an allelic mutant of FLO10, which encodes a mitochondrion-targeted P-type pentatricopeptide repeat protein. Amino acid sequence analysis found that two PPR motifs present in the C-terminal of WBG1 were lost in wbg1. This deletion reduced the splicing efficiency of nad1 intron 1 to approximately 50% in wbg1, thereby partially reducing the activity of complex I and affecting ATP production in wbg1 grains. Furthermore, haplotype analysis showed that WBG1 was associated with grain width between indica and japonica rice varieties. These results suggested that WBG1 influences rice grain chalkiness and grain width by regulating the splicing efficiency of nad1 intron 1. This deepens understanding of the molecular mechanisms governing rice grain quality and provides theoretical support for molecular breeding to improve rice quality. Frontiers Media S.A. 2023-03-09 /pmc/articles/PMC10033517/ /pubmed/36968383 http://dx.doi.org/10.3389/fpls.2023.1136849 Text en Copyright © 2023 Wu, Cai, Zhai, Ye, Zhu, Yu, Ye and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wu, Mingming Cai, Maohong Zhai, Rongrong Ye, Jing Zhu, Guofu Yu, Faming Ye, Shenghai Zhang, Xiaoming A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title | A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title_full | A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title_fullStr | A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title_full_unstemmed | A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title_short | A mitochondrion-associated PPR protein, WBG1, regulates grain chalkiness in rice |
title_sort | mitochondrion-associated ppr protein, wbg1, regulates grain chalkiness in rice |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033517/ https://www.ncbi.nlm.nih.gov/pubmed/36968383 http://dx.doi.org/10.3389/fpls.2023.1136849 |
work_keys_str_mv | AT wumingming amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT caimaohong amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhairongrong amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yejing amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhuguofu amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yufaming amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yeshenghai amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhangxiaoming amitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT wumingming mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT caimaohong mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhairongrong mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yejing mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhuguofu mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yufaming mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT yeshenghai mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice AT zhangxiaoming mitochondrionassociatedpprproteinwbg1regulatesgrainchalkinessinrice |