Cargando…

Off-target effects in CRISPR/Cas9 gene editing

Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Guo, Congting, Ma, Xiaoteng, Gao, Fei, Guo, Yuxuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034092/ https://www.ncbi.nlm.nih.gov/pubmed/36970624 http://dx.doi.org/10.3389/fbioe.2023.1143157

Ejemplares similares

Efficient Single-Gene and Gene Family Editing in the Apicomplexan Parasite Eimeria tenella Using CRISPR-Cas9
por: Hu, Dandan, et al.
Publicado: (2020)

CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila
por: Hu, Mengyi, et al.
Publicado: (2023)

The application of CRISPR /Cas mediated gene editing in synthetic biology: Challenges and optimizations
por: Li, Wenqian, et al.
Publicado: (2022)

Generation of Gene-Knockout Mongolian Gerbils via CRISPR/Cas9 System
por: Wang, Yan, et al.
Publicado: (2020)

CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus
por: Yadavalli, Raghavendra, et al.
Publicado: (2021)

Diosgenin enhances liposome-enabled nucleic acid delivery and CRISPR/Cas9-mediated gene editing by modulating endocytic pathways
por: Lohchania, Brijesh, et al.
Publicado: (2023)

CRISPR-Cas12a-Assisted Genome Editing in Amycolatopsis mediterranei
por: Zhou, Yajuan, et al.
Publicado: (2020)

Editorial: Current approaches to CRISPR/Cas9 delivery
por: Uludağ, Hasan, et al.
Publicado: (2022)

Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing
por: Burghardt, Jan Philipp, et al.
Publicado: (2020)

Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems
por: Zhang, Jun, et al.
Publicado: (2020)

Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology
por: Zhang, Shangjie, et al.
Publicado: (2020)

Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation
por: Foley, Ruth A., et al.
Publicado: (2022)

Cell Reprogramming With CRISPR/Cas9 Based Transcriptional Regulation Systems
por: Shakirova, Ksenia M., et al.
Publicado: (2020)

CRISPR/Cas9 Systems for the Development of Saccharomyces cerevisiae Cell Factories
por: Meng, Jie, et al.
Publicado: (2020)

CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV
por: Dong, Zhanqi, et al.
Publicado: (2020)

CRISPR/Cas systems: Delivery and application in gene therapy
por: Huang, Jie, et al.
Publicado: (2022)

Efficient Genome Editing by a Miniature CRISPR-AsCas12f1 Nuclease in Bacillus anthracis
por: Wang, Yanchun, et al.
Publicado: (2022)

Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease
por: Gopal, Sneha, et al.
Publicado: (2020)

CaSilico: A versatile CRISPR package for in silico CRISPR RNA designing for Cas12, Cas13, and Cas14
por: Asadbeigi, Adnan, et al.
Publicado: (2022)

CRISPR/Cas9-Based Deletion of SpvB Gene From Salmonella gallinarum Leads to Loss of Virulence in Chicken
por: Basit, Abdul, et al.
Publicado: (2022)

Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
por: Xiaoshuai, Li, et al.
Publicado: (2022)

Calcium phosphate-based nanomedicine mediated CRISPR/Cas9 delivery for prostate cancer therapy
por: Wei, Chao-Gang, et al.
Publicado: (2022)

Reprogramming Microbial CO(2)-Metabolizing Chassis With CRISPR-Cas Systems
por: Yu, Hai-Yan, et al.
Publicado: (2022)

Mitigating off-target effects in CRISPR/Cas9-mediated in vivo gene editing
por: Han, Hua Alexander, et al.
Publicado: (2020)

Development and Application of CRISPR/Cas in Microbial Biotechnology
por: Ding, Wentao, et al.
Publicado: (2020)

CRISPR/Cas Technologies and Their Applications in Escherichia coli
por: Dong, Huina, et al.
Publicado: (2021)

CRISPR/Cas13d-Mediated Microbial RNA Knockdown
por: Zhang, Kun, et al.
Publicado: (2020)

Construction of a Stable and Temperature-Responsive Yeast Cell Factory for Crocetin Biosynthesis Using CRISPR-Cas9
por: Liu, Tengfei, et al.
Publicado: (2020)

Optimizing sgRNA to Improve CRISPR/Cas9 Knockout Efficiency: Special Focus on Human and Animal Cell
por: Shojaei Baghini, Sadegh, et al.
Publicado: (2021)

Programmable RNA-Guided Large DNA Transgenesis by CRISPR/Cas9 and Site-Specific Integrase Bxb1
por: Hosur, Vishnu, et al.
Publicado: (2022)

Genetic engineering to enhance microalgal-based produced water treatment with emphasis on CRISPR/Cas9: A review
por: Hassanien, Alaa, et al.
Publicado: (2023)

Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae
por: Feng, Qing, et al.
Publicado: (2023)

Multi-Layer Controls of Cas9 Activity Coupled With ATP Synthase Over-Expression for Efficient Genome Editing in Streptomyces
por: Wang, Kai, et al.
Publicado: (2019)

Retraction: Optimizing sgRNA to improve CRISPR/Cas9 knockout efficiency: special focus on human and animal cell
Publicado: (2023)

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations
por: Seys, François M., et al.
Publicado: (2023)

Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications
por: Hertel, Oliver, et al.
Publicado: (2022)

Bio-SCAN V2: A CRISPR/dCas9-based lateral flow assay for rapid detection of theophylline
por: Jiang, Wenjun, et al.
Publicado: (2023)

Off-target predictions in CRISPR-Cas9 gene editing using deep learning
por: Lin, Jiecong, et al.
Publicado: (2018)

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering
por: Zheng, Yanli, et al.
Publicado: (2020)

CRISPR/Cas9-Mediated Whole Genomic Wide Knockout Screening Identifies Specific Genes Associated With PM(2.5)-Induced Mineral Absorption in Liver Toxicity
por: Peng, Jinfu, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_qnrnjmhvrjvqfkg8a11dr7sai6