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A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17

This manuscript describes a unique protocol for the rapid transformation of Medicago truncatula A17 cell suspension cultures mediated by Agrobacterium tumefaciens. Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then...

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Detalles Bibliográficos
Autores principales: Ferreira, Ana Clara, Rebelo, Bárbara A., Abranches, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034231/
https://www.ncbi.nlm.nih.gov/pubmed/37197004
http://dx.doi.org/10.1007/s11240-023-02495-6