Protocol to test the utility of detergents for E. coli membrane protein extraction and delipidation

We present a protocol to evaluate the utility of detergents for purification and delipidation of E. coli membrane proteins. We determine the critical aggregation concentration of detergents. Furthermore, we compare the ability of detergents to extract membrane proteins and to maintain protein-lipid...

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Detalles Bibliográficos
Autores principales: Wycisk, Virginia, Urner, Leonhard H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034499/
https://www.ncbi.nlm.nih.gov/pubmed/36934326
http://dx.doi.org/10.1016/j.xpro.2023.102146

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034499/
https://www.ncbi.nlm.nih.gov/pubmed/36934326
http://dx.doi.org/10.1016/j.xpro.2023.102146

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