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Protocol to test the utility of detergents for E. coli membrane protein extraction and delipidation
We present a protocol to evaluate the utility of detergents for purification and delipidation of E. coli membrane proteins. We determine the critical aggregation concentration of detergents. Furthermore, we compare the ability of detergents to extract membrane proteins and to maintain protein-lipid...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034499/ https://www.ncbi.nlm.nih.gov/pubmed/36934326 http://dx.doi.org/10.1016/j.xpro.2023.102146 |