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First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection

A double‐stranded RNA (dsRNA) phage phiYY is able to kill a pyomelanin‐producing Pseudomonas aeruginosa strain, which was isolated from a 40‐year‐old man with interstitial lung disease (ILD) and chronic lung infection. Phage therapy was used as a last resort for this patient. The three‐course nebuli...

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Autores principales: Li, Linlin, Zhong, Qiu, Zhao, Yunze, Bao, Juan, Liu, Bing, Zhong, Zhuojun, Wang, Jing, Yang, Lan, Zhang, Tingting, Cheng, Mengjun, Wu, Nannan, Zhu, Tongyu, Le, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034620/
https://www.ncbi.nlm.nih.gov/pubmed/36636832
http://dx.doi.org/10.1111/1751-7915.14217
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author Li, Linlin
Zhong, Qiu
Zhao, Yunze
Bao, Juan
Liu, Bing
Zhong, Zhuojun
Wang, Jing
Yang, Lan
Zhang, Tingting
Cheng, Mengjun
Wu, Nannan
Zhu, Tongyu
Le, Shuai
author_facet Li, Linlin
Zhong, Qiu
Zhao, Yunze
Bao, Juan
Liu, Bing
Zhong, Zhuojun
Wang, Jing
Yang, Lan
Zhang, Tingting
Cheng, Mengjun
Wu, Nannan
Zhu, Tongyu
Le, Shuai
author_sort Li, Linlin
collection PubMed
description A double‐stranded RNA (dsRNA) phage phiYY is able to kill a pyomelanin‐producing Pseudomonas aeruginosa strain, which was isolated from a 40‐year‐old man with interstitial lung disease (ILD) and chronic lung infection. Phage therapy was used as a last resort for this patient. The three‐course nebulized phiYY treatment was used to reduce the bacterial burden and clinical symptoms of the patient. Recurrences of P. aeruginosa infections were observed 1–3 days post phage therapy. The recurrent isolates exhibited distinct antibiotic‐susceptibility profiles compared with the original strain yet were still susceptible to phiYY. This assay represents the application of dsRNA phage in the treatment of chronic lung infection, albeit the safety and efficacy of the dsRNA phage require further assessment.
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spelling pubmed-100346202023-03-24 First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection Li, Linlin Zhong, Qiu Zhao, Yunze Bao, Juan Liu, Bing Zhong, Zhuojun Wang, Jing Yang, Lan Zhang, Tingting Cheng, Mengjun Wu, Nannan Zhu, Tongyu Le, Shuai Microb Biotechnol Brief Report A double‐stranded RNA (dsRNA) phage phiYY is able to kill a pyomelanin‐producing Pseudomonas aeruginosa strain, which was isolated from a 40‐year‐old man with interstitial lung disease (ILD) and chronic lung infection. Phage therapy was used as a last resort for this patient. The three‐course nebulized phiYY treatment was used to reduce the bacterial burden and clinical symptoms of the patient. Recurrences of P. aeruginosa infections were observed 1–3 days post phage therapy. The recurrent isolates exhibited distinct antibiotic‐susceptibility profiles compared with the original strain yet were still susceptible to phiYY. This assay represents the application of dsRNA phage in the treatment of chronic lung infection, albeit the safety and efficacy of the dsRNA phage require further assessment. John Wiley and Sons Inc. 2023-01-13 /pmc/articles/PMC10034620/ /pubmed/36636832 http://dx.doi.org/10.1111/1751-7915.14217 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Li, Linlin
Zhong, Qiu
Zhao, Yunze
Bao, Juan
Liu, Bing
Zhong, Zhuojun
Wang, Jing
Yang, Lan
Zhang, Tingting
Cheng, Mengjun
Wu, Nannan
Zhu, Tongyu
Le, Shuai
First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title_full First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title_fullStr First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title_full_unstemmed First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title_short First‐in‐human application of double‐stranded RNA bacteriophage in the treatment of pulmonary Pseudomonas aeruginosa infection
title_sort first‐in‐human application of double‐stranded rna bacteriophage in the treatment of pulmonary pseudomonas aeruginosa infection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034620/
https://www.ncbi.nlm.nih.gov/pubmed/36636832
http://dx.doi.org/10.1111/1751-7915.14217
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