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In silico evaluation and selection of the best 16S rRNA gene primers for use in next-generation sequencing to detect oral bacteria and archaea

BACKGROUND: Sequencing has been widely used to study the composition of the oral microbiome present in various health conditions. The extent of the coverage of the 16S rRNA gene primers employed for this purpose has not, however, been evaluated in silico using oral-specific databases. This paper ana...

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Detalles Bibliográficos
Autores principales: Regueira-Iglesias, Alba, Vázquez-González, Lara, Balsa-Castro, Carlos, Vila-Blanco, Nicolás, Blanco-Pintos, Triana, Tamames, Javier, Carreira, Maria José, Tomás, Inmaculada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035280/
https://www.ncbi.nlm.nih.gov/pubmed/36949474
http://dx.doi.org/10.1186/s40168-023-01481-6