Cargando…
In silico evaluation and selection of the best 16S rRNA gene primers for use in next-generation sequencing to detect oral bacteria and archaea
BACKGROUND: Sequencing has been widely used to study the composition of the oral microbiome present in various health conditions. The extent of the coverage of the 16S rRNA gene primers employed for this purpose has not, however, been evaluated in silico using oral-specific databases. This paper ana...
Autores principales: | Regueira-Iglesias, Alba, Vázquez-González, Lara, Balsa-Castro, Carlos, Vila-Blanco, Nicolás, Blanco-Pintos, Triana, Tamames, Javier, Carreira, Maria José, Tomás, Inmaculada |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035280/ https://www.ncbi.nlm.nih.gov/pubmed/36949474 http://dx.doi.org/10.1186/s40168-023-01481-6 |
Ejemplares similares
-
Impact of 16S rRNA Gene Redundancy and Primer Pair Selection on the Quantification and Classification of Oral Microbiota in Next-Generation Sequencing
por: Regueira-Iglesias, Alba, et al.
Publicado: (2023) -
In-Silico Detection of Oral Prokaryotic Species With Highly Similar 16S rRNA Sequence Segments Using Different Primer Pairs
por: Regueira-Iglesias, Alba, et al.
Publicado: (2022) -
Short-term anti-plaque effect of a cymenol mouthwash analysed using the DenTiUS Deep Plaque software: a randomised clinical trial
por: Suárez-Rodríguez, B, et al.
Publicado: (2023) -
DenTiUS Plaque, a Web-Based Application for the Quantification of Bacterial Plaque: Development and Usability Study
por: Vila-Blanco, Nicolás, et al.
Publicado: (2020) -
Characterizing the fecal bacteria and archaea community of heifers and lactating cows through 16S rRNA next-generation sequencing
por: Cendron, Filippo, et al.
Publicado: (2020)