Fast, cheap and sensitive: Homogenizer-based RNA extraction free method for SARS-CoV-2 detection by RT-qPCR

BACKGROUND: The SARS-CoV-2 gold standard detection method is an RT-qPCR with a previous step of viral RNA extraction from the patient sample either by using commercial automatized or manual extraction kits. This RNA extraction step is expensive and time demanding. OBJECTIVE: The aim of our study was...

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Detalles Bibliográficos
Autores principales: Ramírez-Córdova, Cristina, Morales-Jadán, Diana, Alarcón-Salem, Sofía, Sarmiento-Alvarado, Alisson, Proaño, María Belén, Camposano, Isabel, Sarmiento-Alvarado, Berenice, Bravo-Castro, Mishell, Hidalgo-Jiménez, Jean Franco, Coello, Dayana, Rodríguez, Ángel S., Viteri-Dávila, Carolina, Vallejo-Janeta, Alexander Paolo, Arcos-Suárez, Daniela, Garcia-Bereguiain, Miguel Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035754/
https://www.ncbi.nlm.nih.gov/pubmed/36968109
http://dx.doi.org/10.3389/fcimb.2023.1074953

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035754/
https://www.ncbi.nlm.nih.gov/pubmed/36968109
http://dx.doi.org/10.3389/fcimb.2023.1074953

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