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Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens
High dropping moisture (DM) in poultry production has deleterious effects on the environment, feeding cost, and public health of people and animals. To explore the contributing genetic components, we classified DM of 67-wk-old Rhode Island Red (RIR) hens at 4 different levels and evaluated the under...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036737/ https://www.ncbi.nlm.nih.gov/pubmed/36931071 http://dx.doi.org/10.1016/j.psj.2022.102242 |
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author | Zhang, Tongyu Zhu, Tao Wen, Junhui Chen, Yu Wang, Liang Lv, Xueze Yang, Weifang Jia, Yaxiong Qu, Changqing Li, Haiying Wang, Huie Qu, Lujiang Ning, Zhonghua |
author_facet | Zhang, Tongyu Zhu, Tao Wen, Junhui Chen, Yu Wang, Liang Lv, Xueze Yang, Weifang Jia, Yaxiong Qu, Changqing Li, Haiying Wang, Huie Qu, Lujiang Ning, Zhonghua |
author_sort | Zhang, Tongyu |
collection | PubMed |
description | High dropping moisture (DM) in poultry production has deleterious effects on the environment, feeding cost, and public health of people and animals. To explore the contributing genetic components, we classified DM of 67-wk-old Rhode Island Red (RIR) hens at 4 different levels and evaluated the underlying genetic heritability. We found the heritability of DM to be 0.219, indicating a moderately heritable trait. We then selected chickens with the highest and lowest DM levels. Using transcriptome, we only detected 12 differentially expressed genes (DEGs) between these 2 groups from the spleen, and 1,507 DEGs from intestinal tissues (jejunum and cecum). The low number of DEGs observed in the spleen suggests that differing moisture levels are not attributed to pathogenic infection. Fourteen of the intestinal high expressed genes are associated with water-salt metabolism (WSM). We also investigated the gut microbial composition by 16S rRNA gene amplicon sequencing. Six different microbial operational taxonomic units (OTUs) (Cetobacterium, Sterolibacterium, Elusimicrobium, Roseburia, Faecalicoccus, and Megamonas) between the 2 groups from jejunum and cecum are potentially biomarkers related to DM levels. Our results identify a genetic component to chicken DM, and can guide breeding strategies. |
format | Online Article Text |
id | pubmed-10036737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100367372023-03-25 Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens Zhang, Tongyu Zhu, Tao Wen, Junhui Chen, Yu Wang, Liang Lv, Xueze Yang, Weifang Jia, Yaxiong Qu, Changqing Li, Haiying Wang, Huie Qu, Lujiang Ning, Zhonghua Poult Sci MICROBIOLOGY AND FOOD SAFETY High dropping moisture (DM) in poultry production has deleterious effects on the environment, feeding cost, and public health of people and animals. To explore the contributing genetic components, we classified DM of 67-wk-old Rhode Island Red (RIR) hens at 4 different levels and evaluated the underlying genetic heritability. We found the heritability of DM to be 0.219, indicating a moderately heritable trait. We then selected chickens with the highest and lowest DM levels. Using transcriptome, we only detected 12 differentially expressed genes (DEGs) between these 2 groups from the spleen, and 1,507 DEGs from intestinal tissues (jejunum and cecum). The low number of DEGs observed in the spleen suggests that differing moisture levels are not attributed to pathogenic infection. Fourteen of the intestinal high expressed genes are associated with water-salt metabolism (WSM). We also investigated the gut microbial composition by 16S rRNA gene amplicon sequencing. Six different microbial operational taxonomic units (OTUs) (Cetobacterium, Sterolibacterium, Elusimicrobium, Roseburia, Faecalicoccus, and Megamonas) between the 2 groups from jejunum and cecum are potentially biomarkers related to DM levels. Our results identify a genetic component to chicken DM, and can guide breeding strategies. Elsevier 2022-10-13 /pmc/articles/PMC10036737/ /pubmed/36931071 http://dx.doi.org/10.1016/j.psj.2022.102242 Text en © 2023 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MICROBIOLOGY AND FOOD SAFETY Zhang, Tongyu Zhu, Tao Wen, Junhui Chen, Yu Wang, Liang Lv, Xueze Yang, Weifang Jia, Yaxiong Qu, Changqing Li, Haiying Wang, Huie Qu, Lujiang Ning, Zhonghua Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title | Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title_full | Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title_fullStr | Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title_full_unstemmed | Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title_short | Gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
title_sort | gut microbiota and transcriptome analysis reveals a genetic component to dropping moisture in chickens |
topic | MICROBIOLOGY AND FOOD SAFETY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036737/ https://www.ncbi.nlm.nih.gov/pubmed/36931071 http://dx.doi.org/10.1016/j.psj.2022.102242 |
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