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Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other tech...

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Detalles Bibliográficos
Autores principales: Labora, Amanda, Lee, Hailey, Chan, Charlotte, Tabornal, Erin, Le, Thuc, Rashid, Khalid, Abt, Evan, Yamao, Takanobu, Mandl, Hanna, Creech, Amanda, Premji, Alykhan, Li, Luyi, Link, Jason, Wu, Nanping, Radu, Caius, Donahue, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036864/
https://www.ncbi.nlm.nih.gov/pubmed/36930646
http://dx.doi.org/10.1016/j.xpro.2023.102163