Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other tech...

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Autores principales: Labora, Amanda, Lee, Hailey, Chan, Charlotte, Tabornal, Erin, Le, Thuc, Rashid, Khalid, Abt, Evan, Yamao, Takanobu, Mandl, Hanna, Creech, Amanda, Premji, Alykhan, Li, Luyi, Link, Jason, Wu, Nanping, Radu, Caius, Donahue, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036864/
https://www.ncbi.nlm.nih.gov/pubmed/36930646
http://dx.doi.org/10.1016/j.xpro.2023.102163
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author Labora, Amanda
Lee, Hailey
Chan, Charlotte
Tabornal, Erin
Le, Thuc
Rashid, Khalid
Abt, Evan
Yamao, Takanobu
Mandl, Hanna
Creech, Amanda
Premji, Alykhan
Li, Luyi
Link, Jason
Wu, Nanping
Radu, Caius
Donahue, Timothy
author_facet Labora, Amanda
Lee, Hailey
Chan, Charlotte
Tabornal, Erin
Le, Thuc
Rashid, Khalid
Abt, Evan
Yamao, Takanobu
Mandl, Hanna
Creech, Amanda
Premji, Alykhan
Li, Luyi
Link, Jason
Wu, Nanping
Radu, Caius
Donahue, Timothy
author_sort Labora, Amanda
collection PubMed
description Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging.
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spelling pubmed-100368642023-03-25 Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection Labora, Amanda Lee, Hailey Chan, Charlotte Tabornal, Erin Le, Thuc Rashid, Khalid Abt, Evan Yamao, Takanobu Mandl, Hanna Creech, Amanda Premji, Alykhan Li, Luyi Link, Jason Wu, Nanping Radu, Caius Donahue, Timothy STAR Protoc Protocol Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the other technique is device-assisted using a 3D-printed prototype device. Finally, we describe tumor surveillance with bioluminescent imaging. Elsevier 2023-03-16 /pmc/articles/PMC10036864/ /pubmed/36930646 http://dx.doi.org/10.1016/j.xpro.2023.102163 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Labora, Amanda
Lee, Hailey
Chan, Charlotte
Tabornal, Erin
Le, Thuc
Rashid, Khalid
Abt, Evan
Yamao, Takanobu
Mandl, Hanna
Creech, Amanda
Premji, Alykhan
Li, Luyi
Link, Jason
Wu, Nanping
Radu, Caius
Donahue, Timothy
Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title_full Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title_fullStr Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title_full_unstemmed Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title_short Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
title_sort generation of liver metastases in a mouse model using ultrasound-guided intravenous injection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036864/
https://www.ncbi.nlm.nih.gov/pubmed/36930646
http://dx.doi.org/10.1016/j.xpro.2023.102163
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