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Protocol for using serial two-photon tomography to map cell types and cerebrovasculature at single-cell resolution in the whole adult mouse brain

Here, we present a protocol using serial two-photon tomography (STPT) to quantitatively map genetically defined cell types and cerebrovasculature at single-cell resolution across the entire adult mouse brain. We describe the preparation of brain tissue and sample embedding for cell type and vascular...

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Detalles Bibliográficos
Autores principales: Liwang, Josephine K., Bennett, Hannah C., Pi, Hyun-Jae, Kim, Yongsoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037193/
https://www.ncbi.nlm.nih.gov/pubmed/36861829
http://dx.doi.org/10.1016/j.xpro.2023.102048