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Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

[Image: see text] Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to...

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Detalles Bibliográficos
Autores principales: Hecko, Sebastian, Schiefer, Astrid, Badenhorst, Christoffel P. S., Fink, Michael J., Mihovilovic, Marko D., Bornscheuer, Uwe T., Rudroff, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037340/
https://www.ncbi.nlm.nih.gov/pubmed/36853077
http://dx.doi.org/10.1021/acs.chemrev.2c00304