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Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity
[Image: see text] Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to...
Autores principales: | Hecko, Sebastian, Schiefer, Astrid, Badenhorst, Christoffel P. S., Fink, Michael J., Mihovilovic, Marko D., Bornscheuer, Uwe T., Rudroff, Florian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037340/ https://www.ncbi.nlm.nih.gov/pubmed/36853077 http://dx.doi.org/10.1021/acs.chemrev.2c00304 |
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