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CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression
Infusion of natural killer (NK) cells is an attractive therapeutic modality in patients with cancer. However, the activity of NK cells is regulated by several mechanisms operating within solid tumors. Regulatory T (Treg) cells suppress NK cell activity through various mechanisms including deprivatio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038043/ https://www.ncbi.nlm.nih.gov/pubmed/36970070 http://dx.doi.org/10.1080/2162402X.2023.2175517 |
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author | Chen, Ziqing Tong, Le Neo, Shi Yong Li, Shuijie Gao, Jiwei Schlisio, Susanne Lundqvist, Andreas |
author_facet | Chen, Ziqing Tong, Le Neo, Shi Yong Li, Shuijie Gao, Jiwei Schlisio, Susanne Lundqvist, Andreas |
author_sort | Chen, Ziqing |
collection | PubMed |
description | Infusion of natural killer (NK) cells is an attractive therapeutic modality in patients with cancer. However, the activity of NK cells is regulated by several mechanisms operating within solid tumors. Regulatory T (Treg) cells suppress NK cell activity through various mechanisms including deprivation of IL-2 via the IL-2 receptor alpha (CD25). Here, we investigate CD25 expression on NK cells to confer persistence in Treg cells containing solid tumor models of renal cell carcinoma (RCC). Compared with IL-2, stimulation with IL-15 increases the expression of CD25 resulting in enhanced response to IL-2 as evidenced by increased phosphorylation of STAT5. Compared with CD25(dim) NK cells, CD25(bright) NK cells isolated from IL-15 primed NK cells display increased proliferative and metabolic activity as well as increased ability to persist in Treg cells containing RCC tumor spheroids. These results support strategies to enrich for or selectively expand CD25(bright) NK cells for adoptive cellular therapy of NK cells. |
format | Online Article Text |
id | pubmed-10038043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-100380432023-03-25 CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression Chen, Ziqing Tong, Le Neo, Shi Yong Li, Shuijie Gao, Jiwei Schlisio, Susanne Lundqvist, Andreas Oncoimmunology Brief Report Infusion of natural killer (NK) cells is an attractive therapeutic modality in patients with cancer. However, the activity of NK cells is regulated by several mechanisms operating within solid tumors. Regulatory T (Treg) cells suppress NK cell activity through various mechanisms including deprivation of IL-2 via the IL-2 receptor alpha (CD25). Here, we investigate CD25 expression on NK cells to confer persistence in Treg cells containing solid tumor models of renal cell carcinoma (RCC). Compared with IL-2, stimulation with IL-15 increases the expression of CD25 resulting in enhanced response to IL-2 as evidenced by increased phosphorylation of STAT5. Compared with CD25(dim) NK cells, CD25(bright) NK cells isolated from IL-15 primed NK cells display increased proliferative and metabolic activity as well as increased ability to persist in Treg cells containing RCC tumor spheroids. These results support strategies to enrich for or selectively expand CD25(bright) NK cells for adoptive cellular therapy of NK cells. Taylor & Francis 2023-03-22 /pmc/articles/PMC10038043/ /pubmed/36970070 http://dx.doi.org/10.1080/2162402X.2023.2175517 Text en © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Brief Report Chen, Ziqing Tong, Le Neo, Shi Yong Li, Shuijie Gao, Jiwei Schlisio, Susanne Lundqvist, Andreas CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title | CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title_full | CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title_fullStr | CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title_full_unstemmed | CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title_short | CD25(bright) NK cells display superior function and metabolic activity under regulatory T cell-mediated suppression |
title_sort | cd25(bright) nk cells display superior function and metabolic activity under regulatory t cell-mediated suppression |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038043/ https://www.ncbi.nlm.nih.gov/pubmed/36970070 http://dx.doi.org/10.1080/2162402X.2023.2175517 |
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