A CRISPR-del-based pipeline for complete gene knockout in human diploid cells

The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion–deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshift-promoting indels can disrupt gene functions by generation...

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Detalles Bibliográficos
Autores principales: Komori, Takuma, Hata, Shoji, Mabuchi, Akira, Genova, Mariya, Harada, Tomoki, Fukuyama, Masamitsu, Chinen, Takumi, Kitagawa, Daiju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Tools and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038147/
https://www.ncbi.nlm.nih.gov/pubmed/36762651
http://dx.doi.org/10.1242/jcs.260000

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038147/
https://www.ncbi.nlm.nih.gov/pubmed/36762651
http://dx.doi.org/10.1242/jcs.260000

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