Protocol for the identification and expression analysis of a cytoplasmic membrane-localized protein STING

Here, we present a protocol for the detection of the two STING isoforms (erSTING and pmSTING) in human peripheral blood mononuclear cells or mouse splenocytes using Western blot and PCR. We detail steps to construct plasmids encoding each isoform and transfer them into mouse and human cell lines. Fi...

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Detalles Bibliográficos
Autores principales: Shi, Jiaqi, Liu, Caiqi, Luo, Shengnan, Zhang, Yingying, Li, Mingwei, Zhou, Meng, Weng, Mingjiao, Li, Xiaobo, Zheng, Tongsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041549/
https://www.ncbi.nlm.nih.gov/pubmed/36943863
http://dx.doi.org/10.1016/j.xpro.2023.102172

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10041549/
https://www.ncbi.nlm.nih.gov/pubmed/36943863
http://dx.doi.org/10.1016/j.xpro.2023.102172

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