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Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy
The development of CAR-T specific therapy made a revolution in modern oncology. Despite the pronounced therapeutic effects, this novel approach displayed several crucial limitations caused by the complications in pharmacokinetics and pharmacodynamics controls. The presence of the several severe medi...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Pleiades Publishing
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042900/ https://www.ncbi.nlm.nih.gov/pubmed/36653580 http://dx.doi.org/10.1134/S1607672922700041 |
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| author | Kalinin, R. S. Shipunova, V. O. Rubtsov, Y. P. Ukrainskay, V. M. Schulga, A. Konovalova, E. V. Volkov, D. V. Yaroshevich, I. A. Moysenovich, A. M. Belogurov, A. A. Telegin, G. B. Chernov, A. S. Maschan, M. A. Terekhov, S. S. Knorre, V. D. Khurs, E. Gnuchev, N. V. Gabibov, A. G. Deyev, S. M. |
| author_facet | Kalinin, R. S. Shipunova, V. O. Rubtsov, Y. P. Ukrainskay, V. M. Schulga, A. Konovalova, E. V. Volkov, D. V. Yaroshevich, I. A. Moysenovich, A. M. Belogurov, A. A. Telegin, G. B. Chernov, A. S. Maschan, M. A. Terekhov, S. S. Knorre, V. D. Khurs, E. Gnuchev, N. V. Gabibov, A. G. Deyev, S. M. |
| author_sort | Kalinin, R. S. |
| collection | PubMed |
| description | The development of CAR-T specific therapy made a revolution in modern oncology. Despite the pronounced therapeutic effects, this novel approach displayed several crucial limitations caused by the complications in pharmacokinetics and pharmacodynamics controls. The presence of the several severe medical complications of CAR-T therapy initiated a set of attempts aimed to regulate their activity in vivo. We propose to apply the barnase-barstar system to control the cytotoxic antitumor activity of CAR-T cells. To menage the regulation targeting effect of the system we propose to use barstar-modified CAR-T cells together with barnase-based molecules. Barnase was fused with designed ankyrin repeat proteins (DARPins) specific to tumor antigens HER2 (human epidermal growth factor receptor 2) The application of the system demonstrates the pronounced regulatory effects of CAR-T targeting. |
| format | Online Article Text |
| id | pubmed-10042900 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Pleiades Publishing |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-100429002023-03-29 Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy Kalinin, R. S. Shipunova, V. O. Rubtsov, Y. P. Ukrainskay, V. M. Schulga, A. Konovalova, E. V. Volkov, D. V. Yaroshevich, I. A. Moysenovich, A. M. Belogurov, A. A. Telegin, G. B. Chernov, A. S. Maschan, M. A. Terekhov, S. S. Knorre, V. D. Khurs, E. Gnuchev, N. V. Gabibov, A. G. Deyev, S. M. Dokl Biochem Biophys Biochemistry, Biophysics, and Molecular Biology The development of CAR-T specific therapy made a revolution in modern oncology. Despite the pronounced therapeutic effects, this novel approach displayed several crucial limitations caused by the complications in pharmacokinetics and pharmacodynamics controls. The presence of the several severe medical complications of CAR-T therapy initiated a set of attempts aimed to regulate their activity in vivo. We propose to apply the barnase-barstar system to control the cytotoxic antitumor activity of CAR-T cells. To menage the regulation targeting effect of the system we propose to use barstar-modified CAR-T cells together with barnase-based molecules. Barnase was fused with designed ankyrin repeat proteins (DARPins) specific to tumor antigens HER2 (human epidermal growth factor receptor 2) The application of the system demonstrates the pronounced regulatory effects of CAR-T targeting. Pleiades Publishing 2023-01-18 2023 /pmc/articles/PMC10042900/ /pubmed/36653580 http://dx.doi.org/10.1134/S1607672922700041 Text en © The Author(s) 2023, ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2023, Vol. 508, pp. 17–20. © The Author(s), 2023. This article is an open access publication.ISSN 1607-6729, Doklady Biochemistry and Biophysics, 2022. © The Author(s), 2022. This article is an open access publication. https://creativecommons.org/licenses/by/4.0/Open Access.This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Biochemistry, Biophysics, and Molecular Biology Kalinin, R. S. Shipunova, V. O. Rubtsov, Y. P. Ukrainskay, V. M. Schulga, A. Konovalova, E. V. Volkov, D. V. Yaroshevich, I. A. Moysenovich, A. M. Belogurov, A. A. Telegin, G. B. Chernov, A. S. Maschan, M. A. Terekhov, S. S. Knorre, V. D. Khurs, E. Gnuchev, N. V. Gabibov, A. G. Deyev, S. M. Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title | Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title_full | Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title_fullStr | Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title_full_unstemmed | Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title_short | Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy |
| title_sort | barnase-barstar specific interaction regulates car-t cells cytotoxic activity toward malignancy |
| topic | Biochemistry, Biophysics, and Molecular Biology |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042900/ https://www.ncbi.nlm.nih.gov/pubmed/36653580 http://dx.doi.org/10.1134/S1607672922700041 |
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