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Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies

Most edible ectomycorrhizal mushrooms are harvested in forests or controlled tree plantations; examples include truffles, chanterelles, porcinis, saffron milk caps, and matsutake. This study explored recent advances in in vitro ectomycorrhizal cultivation of chanterelles and matsutakes for successfu...

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Autor principal: Yamada, Akiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Mycological Society of Japan 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043572/
https://www.ncbi.nlm.nih.gov/pubmed/37089523
http://dx.doi.org/10.47371/mycosci.2022.08.004
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author Yamada, Akiyoshi
author_facet Yamada, Akiyoshi
author_sort Yamada, Akiyoshi
collection PubMed
description Most edible ectomycorrhizal mushrooms are harvested in forests or controlled tree plantations; examples include truffles, chanterelles, porcinis, saffron milk caps, and matsutake. This study explored recent advances in in vitro ectomycorrhizal cultivation of chanterelles and matsutakes for successful ectomycorrhizal seedling establishment and the subsequent manipulation of these seedlings for efficient fruiting body production. Chanterelle cultivation studies have been limited due to the difficulty of establishing pure cultures. However, once pure cultures were established in the Japanese yellow chanterelle (Cantharellus anzutake), its ectomycorrhizal manipulation produced fruiting bodies under controlled laboratory conditions. As C. anzutake strains have fruited repeatedly under ectomycorrhizal symbiosis with pine and oak seedlings, mating tests for the cross breeding are ongoing issues. As one of the established strains C-23 has full-genome sequence, its application for various type of ectomycorrhizal studies is also expected. By contrast, Tricholoma matsutake fruiting bodies have not yet been produced under controlled conditions, despite successful establishment of ectomycorrhizal seedlings. At present, the shiro structure of ≈1L in volume can be provided in two y incubation with pine hosts under controlled environmental conditions. Therefore, further studies that provides larger shiro on the host root system are desired for the outplantation trial and fruiting.
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spelling pubmed-100435722023-04-20 Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies Yamada, Akiyoshi Mycoscience Review Article Most edible ectomycorrhizal mushrooms are harvested in forests or controlled tree plantations; examples include truffles, chanterelles, porcinis, saffron milk caps, and matsutake. This study explored recent advances in in vitro ectomycorrhizal cultivation of chanterelles and matsutakes for successful ectomycorrhizal seedling establishment and the subsequent manipulation of these seedlings for efficient fruiting body production. Chanterelle cultivation studies have been limited due to the difficulty of establishing pure cultures. However, once pure cultures were established in the Japanese yellow chanterelle (Cantharellus anzutake), its ectomycorrhizal manipulation produced fruiting bodies under controlled laboratory conditions. As C. anzutake strains have fruited repeatedly under ectomycorrhizal symbiosis with pine and oak seedlings, mating tests for the cross breeding are ongoing issues. As one of the established strains C-23 has full-genome sequence, its application for various type of ectomycorrhizal studies is also expected. By contrast, Tricholoma matsutake fruiting bodies have not yet been produced under controlled conditions, despite successful establishment of ectomycorrhizal seedlings. At present, the shiro structure of ≈1L in volume can be provided in two y incubation with pine hosts under controlled environmental conditions. Therefore, further studies that provides larger shiro on the host root system are desired for the outplantation trial and fruiting. The Mycological Society of Japan 2022-09-20 /pmc/articles/PMC10043572/ /pubmed/37089523 http://dx.doi.org/10.47371/mycosci.2022.08.004 Text en 2021, by The Mycological Society of Japan https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access paper distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivative 4.0 international license (CC BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Article
Yamada, Akiyoshi
Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title_full Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title_fullStr Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title_full_unstemmed Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title_short Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
title_sort cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043572/
https://www.ncbi.nlm.nih.gov/pubmed/37089523
http://dx.doi.org/10.47371/mycosci.2022.08.004
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