Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging

Fluorescent molecular probes are very powerful tools that have been generally applied in cell imaging in the research fields of biology, pathology, pharmacology, biochemistry, and medical science. In the last couple of decades, numerous molecular probes endowed with high specificity to particular or...

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Autores principales: Lu, Sha, Dai, Zhiqi, Cui, Yunxi, Kong, De-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046058/
https://www.ncbi.nlm.nih.gov/pubmed/36979572
http://dx.doi.org/10.3390/bios13030360
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author Lu, Sha
Dai, Zhiqi
Cui, Yunxi
Kong, De-Ming
author_facet Lu, Sha
Dai, Zhiqi
Cui, Yunxi
Kong, De-Ming
author_sort Lu, Sha
collection PubMed
description Fluorescent molecular probes are very powerful tools that have been generally applied in cell imaging in the research fields of biology, pathology, pharmacology, biochemistry, and medical science. In the last couple of decades, numerous molecular probes endowed with high specificity to particular organelles have been designed to illustrate intracellular images in more detail at the subcellular level. Nowadays, the development of cell biology has enabled the investigation process to go deeply into cells, even at the molecular level. Therefore, probes that can sketch a particular organelle’s location while responding to certain parameters to evaluate intracellular bioprocesses are under urgent demand. It is significant to understand the basic ideas of organelle properties, as well as the vital substances related to each unique organelle, for the design of probes with high specificity and efficiency. In this review, we summarize representative multifunctional fluorescent molecular probes developed in the last decade. We focus on probes that can specially target nuclei, mitochondria, endoplasmic reticulums, and lysosomes. In each section, we first briefly introduce the significance and properties of different organelles. We then discuss how probes are designed to make them highly organelle-specific. Finally, we also consider how probes are constructed to endow them with additional functions to recognize particular physical/chemical signals of targeted organelles. Moreover, a perspective on the challenges in future applications of highly specific molecular probes in cell imaging is also proposed. We hope that this review can provide researchers with additional conceptual information about developing probes for cell imaging, assisting scientists interested in molecular biology, cell biology, and biochemistry to accelerate their scientific studies.
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spelling pubmed-100460582023-03-29 Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging Lu, Sha Dai, Zhiqi Cui, Yunxi Kong, De-Ming Biosensors (Basel) Review Fluorescent molecular probes are very powerful tools that have been generally applied in cell imaging in the research fields of biology, pathology, pharmacology, biochemistry, and medical science. In the last couple of decades, numerous molecular probes endowed with high specificity to particular organelles have been designed to illustrate intracellular images in more detail at the subcellular level. Nowadays, the development of cell biology has enabled the investigation process to go deeply into cells, even at the molecular level. Therefore, probes that can sketch a particular organelle’s location while responding to certain parameters to evaluate intracellular bioprocesses are under urgent demand. It is significant to understand the basic ideas of organelle properties, as well as the vital substances related to each unique organelle, for the design of probes with high specificity and efficiency. In this review, we summarize representative multifunctional fluorescent molecular probes developed in the last decade. We focus on probes that can specially target nuclei, mitochondria, endoplasmic reticulums, and lysosomes. In each section, we first briefly introduce the significance and properties of different organelles. We then discuss how probes are designed to make them highly organelle-specific. Finally, we also consider how probes are constructed to endow them with additional functions to recognize particular physical/chemical signals of targeted organelles. Moreover, a perspective on the challenges in future applications of highly specific molecular probes in cell imaging is also proposed. We hope that this review can provide researchers with additional conceptual information about developing probes for cell imaging, assisting scientists interested in molecular biology, cell biology, and biochemistry to accelerate their scientific studies. MDPI 2023-03-08 /pmc/articles/PMC10046058/ /pubmed/36979572 http://dx.doi.org/10.3390/bios13030360 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lu, Sha
Dai, Zhiqi
Cui, Yunxi
Kong, De-Ming
Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title_full Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title_fullStr Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title_full_unstemmed Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title_short Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging
title_sort recent development of advanced fluorescent molecular probes for organelle-targeted cell imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046058/
https://www.ncbi.nlm.nih.gov/pubmed/36979572
http://dx.doi.org/10.3390/bios13030360
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