Cargando…
Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis
Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa ‘Crimson Glory’, whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely ap...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048243/ https://www.ncbi.nlm.nih.gov/pubmed/36980964 http://dx.doi.org/10.3390/genes14030692 |
_version_ | 1785014134909173760 |
---|---|
author | Xie, Limei Bai, Xue Zhang, Hao Qiu, Xianqin Jian, Hongying Wang, Qigang Wang, Huichun Feng, Dedang Tang, Kaixue Yan, Huijun |
author_facet | Xie, Limei Bai, Xue Zhang, Hao Qiu, Xianqin Jian, Hongying Wang, Qigang Wang, Huichun Feng, Dedang Tang, Kaixue Yan, Huijun |
author_sort | Xie, Limei |
collection | PubMed |
description | Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa ‘Crimson Glory’, whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely applied in production to maintain quality; however, chilling results in a decrease in aromatic volatiles. To determine the molecular basis underlying the changes in aromatic volatile emissions, we investigated the changes in volatile compounds, DNA methylation patterns, and patterns of the transcriptome in response to chilling temperature. The results demonstrated that chilling roses substantially reduced aromatic volatile emissions. We found that these reductions were correlated with the changes in the methylation status of the promoters and genic regions of the genes involved in volatile biosynthesis. These changes mainly occurred for CHH (H = A, T, or C) which accounted for 51% of the total methylation. Furthermore, transcript levels of scent-related gene Germacrene D synthase (RhGDS), Nudix hydrolase 1 (RhNUDX1), and Phenylacetaldehyde reductase (RhPAR) of roses were strikingly depressed after 24 h at low-temperature and remained low-level after 24 h of recovery at 20 °C. Overall, our findings indicated that epigenetic regulation plays an important role in the chilling tolerance of roses and lays a foundation for practical significance in the production of edible roses. |
format | Online Article Text |
id | pubmed-10048243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100482432023-03-29 Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis Xie, Limei Bai, Xue Zhang, Hao Qiu, Xianqin Jian, Hongying Wang, Qigang Wang, Huichun Feng, Dedang Tang, Kaixue Yan, Huijun Genes (Basel) Article Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa ‘Crimson Glory’, whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely applied in production to maintain quality; however, chilling results in a decrease in aromatic volatiles. To determine the molecular basis underlying the changes in aromatic volatile emissions, we investigated the changes in volatile compounds, DNA methylation patterns, and patterns of the transcriptome in response to chilling temperature. The results demonstrated that chilling roses substantially reduced aromatic volatile emissions. We found that these reductions were correlated with the changes in the methylation status of the promoters and genic regions of the genes involved in volatile biosynthesis. These changes mainly occurred for CHH (H = A, T, or C) which accounted for 51% of the total methylation. Furthermore, transcript levels of scent-related gene Germacrene D synthase (RhGDS), Nudix hydrolase 1 (RhNUDX1), and Phenylacetaldehyde reductase (RhPAR) of roses were strikingly depressed after 24 h at low-temperature and remained low-level after 24 h of recovery at 20 °C. Overall, our findings indicated that epigenetic regulation plays an important role in the chilling tolerance of roses and lays a foundation for practical significance in the production of edible roses. MDPI 2023-03-10 /pmc/articles/PMC10048243/ /pubmed/36980964 http://dx.doi.org/10.3390/genes14030692 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Limei Bai, Xue Zhang, Hao Qiu, Xianqin Jian, Hongying Wang, Qigang Wang, Huichun Feng, Dedang Tang, Kaixue Yan, Huijun Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title | Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title_full | Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title_fullStr | Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title_full_unstemmed | Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title_short | Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis |
title_sort | loss of rose fragrance under chilling stress is associated with changes in dna methylation and volatile biosynthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10048243/ https://www.ncbi.nlm.nih.gov/pubmed/36980964 http://dx.doi.org/10.3390/genes14030692 |
work_keys_str_mv | AT xielimei lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT baixue lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT zhanghao lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT qiuxianqin lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT jianhongying lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT wangqigang lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT wanghuichun lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT fengdedang lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT tangkaixue lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis AT yanhuijun lossofrosefragranceunderchillingstressisassociatedwithchangesindnamethylationandvolatilebiosynthesis |