Collateral activity of the CRISPR/RfxCas13d system in human cells

CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While Cas13 nucleases are capable of degrading both target RNAs and bystander RNAs in vitro and in bacteria, initial studies fail to detect collateral degradation of non-target RNAs in eukaryotic cells. Here we show that...

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Detalles Bibliográficos
Autores principales: Shi, Peiguo, Murphy, Michael R., Aparicio, Alexis O., Kesner, Jordan S., Fang, Zhou, Chen, Ziheng, Trehan, Aditi, Guo, Yang, Wu, Xuebing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049998/
https://www.ncbi.nlm.nih.gov/pubmed/36977923
http://dx.doi.org/10.1038/s42003-023-04708-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049998/
https://www.ncbi.nlm.nih.gov/pubmed/36977923
http://dx.doi.org/10.1038/s42003-023-04708-2

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