Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two

BACKGROUND: In plant genome editing, RNA-guided nucleases such as Cas9 from Streptococcus pyogenes (SpCas9) predominantly induce small insertions or deletions at target sites. This can be used for inactivation of protein-coding genes by frame shift mutations. However, in some cases, it may be advant...

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Detalles Bibliográficos
Autores principales: Ordon, Jana, Kiel, Niklas, Becker, Dieter, Kretschmer, Carola, Schulze-Lefert, Paul, Stuttmann, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053088/
https://www.ncbi.nlm.nih.gov/pubmed/36978193
http://dx.doi.org/10.1186/s13007-023-01010-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053088/
https://www.ncbi.nlm.nih.gov/pubmed/36978193
http://dx.doi.org/10.1186/s13007-023-01010-4

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