Guide-specific loss of efficiency and off-target reduction with Cas9 variants

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High-fidelity Cas9 variants have been developed to reduce the off-target effects of CRISPR systems at a cost of efficiency loss. To systematically evaluate the efficiency and off-target tolerance of Cas9 variants in complex with different single guide RNAs (sgRNAs), we applied high-throughput viabil...

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Detalles Bibliográficos
Autores principales: Zhang, Liang, He, Wei, Fu, Rongjie, Xu, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055116/
https://www.ncbi.nlm.nih.gov/pubmed/36993488
http://dx.doi.org/10.1101/2023.03.16.532856

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055116/
https://www.ncbi.nlm.nih.gov/pubmed/36993488
http://dx.doi.org/10.1101/2023.03.16.532856

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