Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases

Traditional genome-editing reagents such as CRISPR-Cas9 achieve targeted DNA modification by introducing double-strand breaks (DSBs), thereby stimulating localized DNA repair by endogenous cellular repair factors. While highly effective at generating heterogenous knockout mutations, this approach su...

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Detalles Bibliográficos
Autores principales: Lampe, George D., King, Rebeca T., Halpin-Healy, Tyler S., Klompe, Sanne E., Hogan, Marcus I., Vo, Phuc Leo H., Tang, Stephen, Chavez, Alejandro, Sternberg, Samuel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055298/
https://www.ncbi.nlm.nih.gov/pubmed/36993517
http://dx.doi.org/10.1101/2023.03.17.533036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055298/
https://www.ncbi.nlm.nih.gov/pubmed/36993517
http://dx.doi.org/10.1101/2023.03.17.533036

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