Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from Coprinopsis cinerea

A endo-1,4-β-mannanase (CcMan5C) gene was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris, and the recombinant enzyme was purified by Ni-affinity chromatography and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/T...

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Detalles Bibliográficos
Autores principales: Yan, Songling, Duan, Baiyun, Liu, Cuicui, Liu, Guiyou, Kang, Liqin, Sun, Lei, Yi, Lin, Zhang, Zhenqing, Liu, Zhonghua, Yuan, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056200/
https://www.ncbi.nlm.nih.gov/pubmed/36983546
http://dx.doi.org/10.3390/jof9030378

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056200/
https://www.ncbi.nlm.nih.gov/pubmed/36983546
http://dx.doi.org/10.3390/jof9030378

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