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Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from Coprinopsis cinerea

A endo-1,4-β-mannanase (CcMan5C) gene was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris, and the recombinant enzyme was purified by Ni-affinity chromatography and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/T...

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Detalles Bibliográficos
Autores principales:	Yan, Songling, Duan, Baiyun, Liu, Cuicui, Liu, Guiyou, Kang, Liqin, Sun, Lei, Yi, Lin, Zhang, Zhenqing, Liu, Zhonghua, Yuan, Sheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10056200/ https://www.ncbi.nlm.nih.gov/pubmed/36983546 http://dx.doi.org/10.3390/jof9030378

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