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Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)

BACKGROUND: The yeast Komagataella phaffii (Pichia pastoris) is routinely used for heterologous protein expression and is suggested as a model organism for yeast. Despite its importance and application potential, no reference gene for transcript analysis via RT-qPCR assays has been evaluated to date...

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Detalles Bibliográficos
Autores principales: Besleaga, Mihail, Vignolle, Gabriel A., Kopp, Julian, Spadiut, Oliver, Mach, Robert L., Mach-Aigner, Astrid R., Zimmermann, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061771/
https://www.ncbi.nlm.nih.gov/pubmed/36991508
http://dx.doi.org/10.1186/s40694-023-00154-1