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Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)
BACKGROUND: The yeast Komagataella phaffii (Pichia pastoris) is routinely used for heterologous protein expression and is suggested as a model organism for yeast. Despite its importance and application potential, no reference gene for transcript analysis via RT-qPCR assays has been evaluated to date...
Autores principales: | Besleaga, Mihail, Vignolle, Gabriel A., Kopp, Julian, Spadiut, Oliver, Mach, Robert L., Mach-Aigner, Astrid R., Zimmermann, Christian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061771/ https://www.ncbi.nlm.nih.gov/pubmed/36991508 http://dx.doi.org/10.1186/s40694-023-00154-1 |
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