Prime editing with genuine Cas9 nickases minimizes unwanted indels

Unlike CRISPR-Cas9 nucleases, which yield DNA double-strand breaks (DSBs), Cas9 nickases (nCas9s), which are created by replacing key catalytic amino-acid residues in one of the two nuclease domains of S. pyogenesis Cas9 (SpCas9), produce nicks or single-strand breaks. Two SpCas9 variants, namely, n...

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Detalles Bibliográficos
Autores principales: Lee, Jaesuk, Lim, Kayeong, Kim, Annie, Mok, Young Geun, Chung, Eugene, Cho, Sung-Ik, Lee, Ji Min, Kim, Jin-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063541/
https://www.ncbi.nlm.nih.gov/pubmed/36997524
http://dx.doi.org/10.1038/s41467-023-37507-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063541/
https://www.ncbi.nlm.nih.gov/pubmed/36997524
http://dx.doi.org/10.1038/s41467-023-37507-8

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