Cargando…

Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications

We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuen Jr, John Se Kit, Saad, Michael K, Xiang, Ning, Barrick, Brigid M, DiCindio, Hailey, Li, Chunmei, Zhang, Sabrina W, Rittenberg, Miriam, Lew, Emily T, Zhang, Kevin Lin, Leung, Glenn, Pietropinto, Jaymie A, Kaplan, David L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072877/
https://www.ncbi.nlm.nih.gov/pubmed/37014056
http://dx.doi.org/10.7554/eLife.82120
_version_ 1785019473406722048
author Yuen Jr, John Se Kit
Saad, Michael K
Xiang, Ning
Barrick, Brigid M
DiCindio, Hailey
Li, Chunmei
Zhang, Sabrina W
Rittenberg, Miriam
Lew, Emily T
Zhang, Kevin Lin
Leung, Glenn
Pietropinto, Jaymie A
Kaplan, David L
author_facet Yuen Jr, John Se Kit
Saad, Michael K
Xiang, Ning
Barrick, Brigid M
DiCindio, Hailey
Li, Chunmei
Zhang, Sabrina W
Rittenberg, Miriam
Lew, Emily T
Zhang, Kevin Lin
Leung, Glenn
Pietropinto, Jaymie A
Kaplan, David L
author_sort Yuen Jr, John Se Kit
collection PubMed
description We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production.
format Online
Article
Text
id pubmed-10072877
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-100728772023-04-05 Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications Yuen Jr, John Se Kit Saad, Michael K Xiang, Ning Barrick, Brigid M DiCindio, Hailey Li, Chunmei Zhang, Sabrina W Rittenberg, Miriam Lew, Emily T Zhang, Kevin Lin Leung, Glenn Pietropinto, Jaymie A Kaplan, David L eLife Cell Biology We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production. eLife Sciences Publications, Ltd 2023-04-04 /pmc/articles/PMC10072877/ /pubmed/37014056 http://dx.doi.org/10.7554/eLife.82120 Text en © 2023, Yuen Jr et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Yuen Jr, John Se Kit
Saad, Michael K
Xiang, Ning
Barrick, Brigid M
DiCindio, Hailey
Li, Chunmei
Zhang, Sabrina W
Rittenberg, Miriam
Lew, Emily T
Zhang, Kevin Lin
Leung, Glenn
Pietropinto, Jaymie A
Kaplan, David L
Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title_full Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title_fullStr Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title_full_unstemmed Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title_short Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
title_sort aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072877/
https://www.ncbi.nlm.nih.gov/pubmed/37014056
http://dx.doi.org/10.7554/eLife.82120
work_keys_str_mv AT yuenjrjohnsekit aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT saadmichaelk aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT xiangning aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT barrickbrigidm aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT dicindiohailey aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT lichunmei aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT zhangsabrinaw aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT rittenbergmiriam aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT lewemilyt aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT zhangkevinlin aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT leungglenn aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT pietropintojaymiea aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications
AT kaplandavidl aggregatinginvitrogrownadipocytestoproducemacroscalecellculturedfattissuewithtunablelipidcompositionsforfoodapplications