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Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072877/ https://www.ncbi.nlm.nih.gov/pubmed/37014056 http://dx.doi.org/10.7554/eLife.82120 |
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author | Yuen Jr, John Se Kit Saad, Michael K Xiang, Ning Barrick, Brigid M DiCindio, Hailey Li, Chunmei Zhang, Sabrina W Rittenberg, Miriam Lew, Emily T Zhang, Kevin Lin Leung, Glenn Pietropinto, Jaymie A Kaplan, David L |
author_facet | Yuen Jr, John Se Kit Saad, Michael K Xiang, Ning Barrick, Brigid M DiCindio, Hailey Li, Chunmei Zhang, Sabrina W Rittenberg, Miriam Lew, Emily T Zhang, Kevin Lin Leung, Glenn Pietropinto, Jaymie A Kaplan, David L |
author_sort | Yuen Jr, John Se Kit |
collection | PubMed |
description | We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production. |
format | Online Article Text |
id | pubmed-10072877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-100728772023-04-05 Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications Yuen Jr, John Se Kit Saad, Michael K Xiang, Ning Barrick, Brigid M DiCindio, Hailey Li, Chunmei Zhang, Sabrina W Rittenberg, Miriam Lew, Emily T Zhang, Kevin Lin Leung, Glenn Pietropinto, Jaymie A Kaplan, David L eLife Cell Biology We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat tissue is produced by mechanically harvesting and aggregating the lipid-filled adipocytes into 3D constructs using alginate or transglutaminase binders. The 3D fat tissues were visually similar to fat tissue harvested from animals, with matching textures based on uniaxial compression tests. The mechanical properties of cultured fat tissues were based on binder choice and concentration, and changes in the fatty acid compositions of cellular triacylglyceride and phospholipids were observed after lipid supplementation (soybean oil) during in vitro culture. This approach of aggregating individual adipocytes into a bulk 3D tissue provides a scalable and versatile strategy to produce cultured fat tissue for food-related applications, thereby addressing a key obstacle in cultivated meat production. eLife Sciences Publications, Ltd 2023-04-04 /pmc/articles/PMC10072877/ /pubmed/37014056 http://dx.doi.org/10.7554/eLife.82120 Text en © 2023, Yuen Jr et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Yuen Jr, John Se Kit Saad, Michael K Xiang, Ning Barrick, Brigid M DiCindio, Hailey Li, Chunmei Zhang, Sabrina W Rittenberg, Miriam Lew, Emily T Zhang, Kevin Lin Leung, Glenn Pietropinto, Jaymie A Kaplan, David L Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title | Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title_full | Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title_fullStr | Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title_full_unstemmed | Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title_short | Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
title_sort | aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10072877/ https://www.ncbi.nlm.nih.gov/pubmed/37014056 http://dx.doi.org/10.7554/eLife.82120 |
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