Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system

DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET...

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Detalles Bibliográficos
Autores principales: Zheng, Yunxi, He, Reqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074173/
https://www.ncbi.nlm.nih.gov/pubmed/37033707
http://dx.doi.org/10.17912/micropub.biology.000814
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author Zheng, Yunxi
He, Reqing
author_facet Zheng, Yunxi
He, Reqing
author_sort Zheng, Yunxi
collection PubMed
description DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd) and a SunTag system. The SunTag-dCpf1-TET1cd system is able to achieve targeted DNA demethylation and up-regulate gene expression when guided to the FWA or CACTA1 loci in Arabidopsis thaliana . Our study provides tools for targeted removal of DNA cytosine methylation, and activation of protein-coding genes or transposons expression.
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spelling pubmed-100741732023-04-06 Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system Zheng, Yunxi He, Reqing MicroPubl Biol New Methods DNA methylation is a stable and heritable epigenetic mark, and it plays an important role in regulation of gene expression and transposon silencing. Here we developed a CRISPR/dCpf1-based targeted demethylation system using the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd) and a SunTag system. The SunTag-dCpf1-TET1cd system is able to achieve targeted DNA demethylation and up-regulate gene expression when guided to the FWA or CACTA1 loci in Arabidopsis thaliana . Our study provides tools for targeted removal of DNA cytosine methylation, and activation of protein-coding genes or transposons expression. Caltech Library 2023-03-20 /pmc/articles/PMC10074173/ /pubmed/37033707 http://dx.doi.org/10.17912/micropub.biology.000814 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Zheng, Yunxi
He, Reqing
Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title_full Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title_fullStr Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title_full_unstemmed Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title_short Targeted DNA demethylation of the Arabidopsis genome using the SunTag-dCpf1-TET1cd system
title_sort targeted dna demethylation of the arabidopsis genome using the suntag-dcpf1-tet1cd system
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074173/
https://www.ncbi.nlm.nih.gov/pubmed/37033707
http://dx.doi.org/10.17912/micropub.biology.000814
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