An artificial metallolyase with pliable 2-His-1-carboxylate facial triad for stereoselective Michael addition

We repurposed the metal-binding site of a cupin superfamily protein into the 2-His-1-carboxylate facial triad, which is one of the common motifs in natural non-heme enzymes, to construct artificial metalloenzymes that can catalyze new-to-nature reactions. The Cu(2+)-H52A/H58E variant catalyzed the s...

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Detalles Bibliográficos
Autores principales: Matsumoto, Ryusei, Yoshioka, Saho, Yuasa, Miho, Morita, Yoshitsugu, Kurisu, Genji, Fujieda, Nobutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074404/
https://www.ncbi.nlm.nih.gov/pubmed/37035687
http://dx.doi.org/10.1039/d2sc06809e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074404/
https://www.ncbi.nlm.nih.gov/pubmed/37035687
http://dx.doi.org/10.1039/d2sc06809e

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