Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage

Cas12a is one of the most commonly used Cas proteins for genome editing and gene regulation. The first key step for Cas12a to fulfill its function is to search for its target among numerous nonspecific and off-target sites. Cas12a utilizes one-dimensional diffusion along the contour of dsDNA to effi...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Ruirui, Zhao, Yuqian, Wang, Wenjuan, Liu, Jun-Jie Gogo, Chen, Chunlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074435/
https://www.ncbi.nlm.nih.gov/pubmed/37035707
http://dx.doi.org/10.1039/d2sc05463a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10074435/
https://www.ncbi.nlm.nih.gov/pubmed/37035707
http://dx.doi.org/10.1039/d2sc05463a

Ejemplares similares