Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease

Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this stu...

Descripción completa

Detalles Bibliográficos
Autores principales: Qiao, Huarui, Li, Lingyun, Wang, Lu, Yu, Haijun, Hu, Fangzheng, Zhou, Xin, Yang, Hongzhao, Xu, Jianfeng, Meng, Xiangjing, Geng, Yong, Dai, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076250/
https://www.ncbi.nlm.nih.gov/pubmed/37030644
http://dx.doi.org/10.1016/j.pep.2023.106267
_version_ 1785020089989332992
author Qiao, Huarui
Li, Lingyun
Wang, Lu
Yu, Haijun
Hu, Fangzheng
Zhou, Xin
Yang, Hongzhao
Xu, Jianfeng
Meng, Xiangjing
Geng, Yong
Dai, Yuanyuan
author_facet Qiao, Huarui
Li, Lingyun
Wang, Lu
Yu, Haijun
Hu, Fangzheng
Zhou, Xin
Yang, Hongzhao
Xu, Jianfeng
Meng, Xiangjing
Geng, Yong
Dai, Yuanyuan
author_sort Qiao, Huarui
collection PubMed
description Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses.
format Online
Article
Text
id pubmed-10076250
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-100762502023-04-06 Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease Qiao, Huarui Li, Lingyun Wang, Lu Yu, Haijun Hu, Fangzheng Zhou, Xin Yang, Hongzhao Xu, Jianfeng Meng, Xiangjing Geng, Yong Dai, Yuanyuan Protein Expr Purif Article Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses. Elsevier Inc. 2023-07 2023-04-06 /pmc/articles/PMC10076250/ /pubmed/37030644 http://dx.doi.org/10.1016/j.pep.2023.106267 Text en © 2023 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Qiao, Huarui
Li, Lingyun
Wang, Lu
Yu, Haijun
Hu, Fangzheng
Zhou, Xin
Yang, Hongzhao
Xu, Jianfeng
Meng, Xiangjing
Geng, Yong
Dai, Yuanyuan
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title_full Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title_fullStr Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title_full_unstemmed Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title_short Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease
title_sort preparation and characterization of nanobodies targeting sars-cov-2 papain-like protease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076250/
https://www.ncbi.nlm.nih.gov/pubmed/37030644
http://dx.doi.org/10.1016/j.pep.2023.106267
work_keys_str_mv AT qiaohuarui preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT lilingyun preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT wanglu preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT yuhaijun preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT hufangzheng preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT zhouxin preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT yanghongzhao preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT xujianfeng preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT mengxiangjing preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT gengyong preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease
AT daiyuanyuan preparationandcharacterizationofnanobodiestargetingsarscov2papainlikeprotease

Ejemplares similares