Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies

We present a method for human brain fixation based on simultaneous perfusion of 4% paraformaldehyde through carotids after a flush with saline. The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. The aim of our...

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Detalles Bibliográficos
Autores principales: Insausti, Ricardo, Insausti, Ana María, Muñoz López, Mónica, Medina Lorenzo, Isidro, Arroyo-Jiménez, Maria del Mar, Marcos Rabal, María Pilar, de la Rosa-Prieto, Carlos, Delgado-González, José Carlos, Montón Etxeberria, Javier, Cebada-Sánchez, Sandra, Raspeño-García, Juan Francisco, Iñiguez de Onzoño, María Mercedes, Molina Romero, Francisco Javier, Benavides-Piccione, Ruth, Tapia-González, Silvia, Wisse, Laura E. M., Ravikumar, Sadhana, Wolk, David A., DeFelipe, Javier, Yushkevich, Paul, Artacho-Pérula, Emilio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076536/
https://www.ncbi.nlm.nih.gov/pubmed/37034833
http://dx.doi.org/10.3389/fnana.2023.1149674

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10076536/
https://www.ncbi.nlm.nih.gov/pubmed/37034833
http://dx.doi.org/10.3389/fnana.2023.1149674

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